Stereology software2/15/2023 Later, NeuroExplorer software was used to interpret fiber depth information and obtain the three-dimensional pathway of each fiber bundle along the rostrocaudal extension of each section. Additionally, in each screening field of the section, all focal planes were inspected and lines depicted only on focused fibers and/or fiber segments. Using high magnification lens, rat brain sections were screened and lines superimposed with virtually all fibers and terminal boutons present in each brain section. Here we describe the technique in detail using the injection of biotinylated-dextran amine (BDA) in the rat cuneate nucleus and its projection through the pyramidal decussation as an example.Ĭamera-lucida-like computer drawings were produced with StereoInvestigator software. In a recent study we have adapted for the first time available software used in spatial neuronal organization studies to represent the complete fiber distribution of a brain projection.
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